Syllabus for Anatomical Methods (MCBA 740)  Lectures Mondays – 10AM Bldg 1 Room B60

Week 1

Lecture

Intro: Price

  1. Course Requirements
  2. Rules for use of IRF
  3. Specimen fixation, processing, sectioning, H&E and Trichrome stains

Lab

Schedule time in groups of 2 with Benny Davidson for training

  1. Fixation and Processing to get paraffin blocks
  2. Paraffin and Cryo sectioning
  3. H&E and Trichrome stains on tissue of choice

Week 2

Lecture – Price

  1. Antigen:Antibody Interactions, Immunocytochemistry and Histochemistry

Lab

  1. Continue with above lab material – Benny
  2. Use Antigen Retrieval techniques on paraffin slides and stain slides with provided immunohistochemistry kit – Sharon Cooper
  3. Training on light microscopes and image acquisition – Dr. Fuseler

Week 3

No lecture – Labor Day

Lab

  1. Continue with above lab exercises -Davidson
  2. Use of Automated staining and Image Acquisition systems – Sharon Cooper
  3. Photoshop – Jeff Davis
  4. Image Pro Plus – Jeff (Alternatively if you prefer to learn Metamorph you can work with Dr. Fuseler on this program)

Week 4

Lecture – Price

  1. Antigen:Antibody interactions for histochemistry/cytochemistry
  2. Digital Imaging

Lab

  1. Finish with above materials. You should be done with all sectioning, staining and image acquisition exercises by the end of week 4.

Week 5

Lecture - Price

  1. Digital Imaging Continued
  2. Confocal

Lab

  1. Specimen processing for confocal including cells, vibratome sections and triple label staining – Anna Harper

Week 6

Lecture – Price

  1. Confocal Continued

Lab – Price and Anna Harper

  1. Confocal training - Price

Week 7

Lecture – Price

Confocal continued

Lab

Confocal continued

Week 8

Lecture

Confocal continued

Lab

Confocal continued: All confocal work should be finished by the end of Week 8

Week 9: Oct 17 – Oct 23

Lecture – Fuseler

  1. Live cell imaging

Lab – Fuseler

  1. Time lapse imaging
  2. Imaging calcium/pH fluxes

Week 10

Lecture – Price

  1. Scanning and Transmission Electron Microscopy

Lab

1. Finish live cell imaging exercises - Fuseler

2. Start processing specimens for SEM and TEM - Jeff

Week 11

Lecture – Price

  1. Scanning and Transmission Electron Microscopy

Lab – Price, Fuseler and Jeff Davis

  1. Finish processing specimens for SEM and TEM - Jeff
  2. Training on SEM – Jeff

Week 12


Lecture – Cell Ultrastructure

Lab

  1. Image SEM samples – Jeff
  2. Ultramicrotomy demonstration – Price
  3. Staining TEM samples – Jeff

Week 13

Lecture – none

Lab

  1. Training on TEM – Price

Week 14

Lecture – none

Lab

  1. SEM and TEM exercises

Week 15

Finish all labs