Syllabus for Anatomical Methods (ANAT 741) Spring 2011
Lectures Mondays – 10AM – Noon;  Bldg 1 Room B60
Labs to be arranged with Professor teaching each section

Week 1

Lecture

Price: Intro

  1. Introduction to the IRF
  2. Rules for use of IRF
  3. Course Requirements

Potts: RNA techniques: In situ hybridization, Agilent 2100 Bioanalyzer, Real time PCR

Lab

Potts – Schedule time Dr. Potts for training on equipment

Week 2

No lecture – MLK Day

Lab

Continue with above lab material

Week 3

Lecture – Potts

RNA techniques: In situ hybridization, Agilent 2100 Bioanalyzer, Real time PCR

Lab

Continue with above lab exercises

Week 4

Lecture – Potts

RNA techniques: In situ hybridization, Agilent 2100 Bioanalyzer, Real time PCR; Begin protein analysis lectures

Lab

Continue with above lab exercises and schedule time with Dr. Potts for protein analysis exercises

Week 5

Lecture – Potts

Protein Techniques: Western blots, Phosphorimager, Bioanalyzer

Lab

Finish all above exercises for in situ hybridization, Agilent 2100 Bioanalyzer and Real Time PCR

  1. Schedule time with Dr. Potts for protein analysis exercises

Week 6

Lecture - Valarmathi

Stem cell techniques

Lab

Continue with protein analysis and schedule time with Dr. V for stem cell exercises

Week 7

Lecture – Singh

FACS and Cell Sorting Techniques: FC 500, FACS ARIA II, AutoMACS Pro

Lab

Continue with Stem Cell Exercises and schedule time with Dr. Udai Singh to begin cell sorting exercises

Week 8

Lecture – Singh

Cell sorting techniques:

Lab

Continue with Stem Cell and cell sorting exercises

Week 9

Lecture – Singh

Cell sorting techniques

Lab

Continue with and cell sorting exercises

Week 11

Lecture – Singh

Cell sorting techniques

Lab

Continue with Stem Cell and cell sorting exercises

Week 12

Lecture - Singh

Bioplex and cell sorting

Lab

Finish FACS and sorting exercises

  1. Schedule time with Dr. Singh on BioPlex

Week 13

Lecture – Singh

Bioplex and Cell Sorting

Lab – Singh

Continue with experiments on FACS and BioPlex equipment

Week 14

Lecture – Creek

Microarray technology

Lab – Singh and Creek

Continue with experiments on FACS and BioPlex equipment

Schedule time with Dr. Creek for microarray exercises (equipment and lab in Pharmacy on main campus)

Week 15

Lecture – Creek

Microarrays if necessary

Lab – Creek

Continue with experiments on microarray technology

Week 16

No Lecture

Lab

Finish microarray exercises