Electron Microscopy Systems

JEOL 200CX Transmission Electron Microscope

Description- The JEOL 200CX transmission electron microscope is a standard TEM system for imaging biological ultrastructure. The IRF system is equipped with:

  • Tungsten filament
  • Resolution of 0.2nm
  • Side entry eucentric goniometer stage with +/-30 tilt
  • Specimen exchange rod with slots for two 3mm grids
  • Accelerating voltages of 80,100,120,160 and 200 kV
  • Low and high magnification operating modes
  • Film cassette for up to 50 sheets of 3.25 X 4 inch film
  • Advanced Microscopy Techniques side mounted 2K digital camera

Applications-

  • High resolution ultrastructural imaging
  • Immunocytochemistry with collodial gold and other electron dense probes
  • Stereo TEM
  • Diffraction

Location- The JEOL 200CX system is located in the Building 1 Basement lab of the IRF.

JEOL 6300V Scanning Electron Microscope

Description- The JEOL 6300V scanning electron microscope is a fully integrated digital high vacuum system. The system in the IRF is equipped with:

  • Tungsten filiment
  • High resolution secondary and TV rate backscatter detectors
  • Resolution in secondary mode of 3.5nm
  • Resolution in backscattered mode of 10nm
  • Sample size of up to 5 inches in diameter can be fully imaged
  • Motorized X-Y stage that can be driven to stored positions
  • Preset Z working distances from 8, 15, 25, 39, and 48mm
  • Eucentric stage with tilt capablilty of -5 to + 90 at a working distance of 48mm
  • 360 rotation
  • Accelerating voltages from 0.5-30kV
  • Magnifications from 5X-300,000X
  • Visions Software Package
  • Polaroid film and digital image storage

Applications-

  • Imaging surface morphology of biological and materials samples
  • Imaging cell: cell and cell:ECM interactions
  • Colloidal gold immunocytochemistry of surface molecules

Location- The JEOL 6300V system is located in the Building 1 Basement lab of the IRF.

Equipment for Specimen Processing:

The IRF is equipped with a wide range of specimen processing equipment for both scanning and transmission electron microscopy including the necessary centrifuges, vacuum ovens, Tousimis Samdri-PVT-3B critical point dryer, Polaron sputter coater and Leica Ultracut R ultramicrotomes: BioRad SEM Coating System(bottom left), Tousimis Samdri-PVT-3B Critical Point Dryer (upper right) and Leica Ultracut R (right).