Histology and Light Microscopy

Equipment for Specimen Processing — fully equipped histology facility for individual or service processing and sectioning of tissues for light microscopy is available in Building 1 Room B60 of the IRF. Available equipment includes:

  • Citadel 1000 Tissue Processor capable of automated computer controlled processing of up to 50 standard tissue cassettes simultaneously
  • PELCO Biowave microwave processing center with vacuum infiltration and Coldspot attachments for even distribution of microwave heating
  • Triangle Biomedical Sciences Embedding Station with side-by-side platforms for embedding and cooling paraffin blocks
  • Two Reichert-Jung 2030 rotary microtomes
  • Zeiss Microm 505HN Cryostat
  • Vibratome Tissue Slicers

Applications — Equipment and protocols exist within the IRF for virtually all histological applications from routine hematoxylin and eosin staining through more detailed techniques such as immunohistochemistry.

Imaging Systems for Light Microscopy- Several microscopes are available for routine brightfield, darkfield and fluorescence imaging.

  • Nikon E600 Widefield Epifluorescence and Darkfield Microscopy System
    • Description — The Nikon E600 configuration consists of:
    • Excitation and emission filters for UV, green, red and far red dyes
    • Fiber optic darkfield illuminator for high-resolution darkfield imaging
    • Wide range of Plan and Plan-Fluor objectives ranging from 4X through 100X oil
    • Phase and DIC objectives and condensers
    • Micropublisher digital camera with Qimaging software
    • Location- The E600 system is located in the Building 1 Basement lab of the IRF
  • Nikon Optiphot Epifluorescence System
    • Description — The Nikon Optiphot Epifluorescence system configuration consists of:
    • Excitation and emission filters for red/green wide field fluorescence
    • 4,10,40 and 60X oil objectives
    • Zeiss Axiocam MRc digital camera and Axiovision software
    • Location- The Optiphot system is located in Building 1 Room B60.
  • Zeiss SteREO Lumar V.12 Stereomicroscope
    • Description — The Zeiss Lumar V.12 system consists of:
    • Filter sets for fluorescent imaging of EGFP, Rhodamine, CFP, YFP, Cy3 and DAPI ranges of dyes
    • LCD cold light source for transmitted light imaging
    • Fiber optic illumination source
    • Zeiss Axiocam MRm digital camera with Axiovision software
    • Location- The Lumar V.12 system is located in Building 1 Room B60.
    • Website- www.zeiss.com
  • Nikon SMZ 1500 Stereomicroscope

    • Description- The Nikon SMZ 1500 Stereomicroscope system consists of:
    • Filter sets for fluorescent imaging of EGFP and red ranges of dyes
    • Transmitted light imaging
    • Location — The Nikon SMZ 1500 system is located in the Building 1 Basement lab.